nf κb inhibitor Search Results


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Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) <t>PDTC</t> or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, <t>pyrrolidine</t> <t>dithiocarbamate;</t> IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.
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Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) <t>PDTC</t> or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, <t>pyrrolidine</t> <t>dithiocarbamate;</t> IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.
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Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) <t>PDTC</t> or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, <t>pyrrolidine</t> <t>dithiocarbamate;</t> IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.
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Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) <t>PDTC</t> or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, <t>pyrrolidine</t> <t>dithiocarbamate;</t> IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.
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Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) <t>PDTC</t> or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, <t>pyrrolidine</t> <t>dithiocarbamate;</t> IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.
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ChemScene llc nf-κb inhibitor-dhmeq
Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) <t>PDTC</t> or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, <t>pyrrolidine</t> <t>dithiocarbamate;</t> IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.
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Image Search Results


Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) PDTC or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, pyrrolidine dithiocarbamate; IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.

Journal: Oncology Reports

Article Title: TNF-α promotes the malignant transformation of intestinal stem cells through the NF-κB and Wnt/β-catenin signaling pathways

doi: 10.3892/or.2020.7631

Figure Lengend Snippet: Effects of inhibitors of the NF-κB and Wnt/β-catenin pathways on their activities in TNF-α-induced NCM460s cells. NCM460s cells were respectively incubated with 1, 10, 100 µM of (A) PDTC or (B) IWP-2 without or with 1 ng/ml TNF-α for 3 h. Western blotting was used to analyze the protein expression of genes related with NF-κB and Wnt/β-catenin signaling in the total cell extracts and the nuclear/cytoplasmic extracts. TNF, tumor necrosis factor; NCM460s, NCM460 spheroid; PDTC, pyrrolidine dithiocarbamate; IKKα/β, IκB kinase complex α/β; GSK, glycogen synthase kinase.

Article Snippet: The NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC; cat. no. M4005) and Wnt inhibitor IWP-2 (cat. no. M2237) were purchased from Abmole (Abmole Bioscience, Inc.).

Techniques: Incubation, Western Blot, Expressing